DNA extraction and sex determination for human teeth dated 3000 years ago unearthed in Xi'an.
- Author:
Pei-huan WANG
1
;
Jin-ling SHAO
;
Xiao-hong DUAN
;
Yu GAO
;
Dai-yun LIU
Author Information
- Publication Type:Journal Article
- MeSH: Amelogenin; genetics; China; DNA; analysis; genetics; isolation & purification; Female; History, Ancient; Humans; Male; Paleodontology; Polymerase Chain Reaction; Sex Determination Analysis; Tooth; chemistry
- From: Chinese Journal of Medical Genetics 2013;30(5):619-621
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo extracted DNA from ancient human teeth dated 3000 years ago unearthed in Xi'an and determine the genders for the individuals.
METHODSThirty five ancient human teeth were studied. A 'Reverse-root-canal' technique and a Chelex-100 solution were used to extract the DNA. Specific primers for Amelogenin gene were designed for PCR amplification.
RESULTSGenomic DNA was successfully extracted from 30 samples, for which 8 were determined to be males and 22 were females.
CONCLUSIONThe 'Reverse-root-canal' technique may be used for extracting DNA from ancient human teeth. Genetics method can supplement physical anthropology for determination of sex for ancient samples.
