A study of HLA-DPA1 and DPB1 matching status for unrelated donor-recipient pairs matched at allele level for HLA-A, -B, -C, -DRB1 and -DQB1 loci.
- VernacularTitle:HLA-A、-B、-C、-DRB1和-DQB1等位基因全相合的无关供-受者对的HLA-DPA1和-DPB1基因匹配性研究
- Author:
Jian-xin ZHEN
1
;
Hong-yan ZOU
;
Shi-zheng JIN
;
Su-qing GAO
;
Da-ming WANG
;
Liu-mei HE
;
Zhi-hui DENG
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; HLA-DP alpha-Chains; genetics; HLA-DP beta-Chains; genetics; HLA-DQ beta-Chains; genetics; Histocompatibility Antigens Class I; genetics; Histocompatibility Testing; methods; Humans; Transplantation; methods; Unrelated Donors
- From: Chinese Journal of Medical Genetics 2013;30(6):697-700
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the status of HLA-DPA1 and DPB1 matching for unrelated donor-recipient pairs matched at high-resolution allele level for HLA-A, B, C, DRB1 and DQB1 loci.
METHODSA total of 76 unrelated donor-recipient pairs matching at allele level for HLA-A, B, C, DRB1 and DQB1 loci were subjected to HLA-DPA1 and DPB1 sequence-based typing (SBT). HLA-DPA1and DPB1 matching status at high-resolution allelic level was also analyzed.
RESULTSThe allelic identity ratio for single HLA-DPA1 and DPB1 were 17.1% and 9.2%, respectively. HLA-DPA1 and DPB1 allelic identity ratio were both very low. The majority of unrelated donor-recipient pairs (73.7%) had an incompatibility at 1 HLA-DPA1 allele, 9.2% of pairs had an incompatibility at 2 DPA1 alleles. As for the high-polymorphic HLA-DPB1 gene, 57.9% of studied donor-recipient pairs had an incompatibility at 1 HLA-DPB1 allele, almost 1/3 (32.9%) of them were completely incompatible. When HLA-DPA1 and DPB1 genes were analyzed together, the donor-recipient pairs matched at 2/4 was the most common (51.4%), 4/4 allelic complete matched pairs accounted for 5.6%, and 0/4 matched pairs accounted for 8.3%.
CONCLUSIONOur results indicated that the ratio of HLA-DPA1 and DPB1 complete match in the unrelated donor-recipient pairs matching at allelic level for HLA-A, B, C, DRB1 and DQB1 loci were very low. The effect of HLA-DPA1 and DPB1 matching status on clinical unrelated stem cell transplantation still needs to be elucidated.
