Genetic polymorphisms of 10 short tandem repeat loci in Lhasa Tibetans in China.
- VernacularTitle:拉萨藏族10个短串联重复基因座的遗传多态性研究
- Author:
Li YUAN
1
;
Lei-peng SHANG
;
Qin-xiang LIAO
;
Juan GUI
;
Lin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Asian Continental Ancestry Group; genetics; China; Genotype; Heterozygote; Humans; Microsatellite Repeats; Polymorphism, Genetic
- From: Chinese Journal of Medical Genetics 2013;30(6):739-741
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate genetic polymorphisms of 10 short tandem repeats loci (D6S1043, D7S3048, D9S925, D10S2325, D11S2368, D14S608, D15S659, D17S1290, D20S470 and GATA198B05) in Tibetans from Lhasa, China.
METHODSFollowing extraction, DNA from 208 unrelated Tibetan individuals was amplified with a self-designed multiplex PCR system. The amplified fragments were separated by electrophoresis on an ABI 3130 Genetic Analyzer and analyzed with GeneMapper®3.2 software.
RESULTSThe distributions of genotype for the 10 STR loci in the population were in accordance with Hardy-Weinberg equilibrium. The polymorphism information component for the 10 loci was 0.750-0.860, the degree of heterozygosity was 0.726-0.865, the discrimination power was 0.919-0.968, and the probability of exclusion was 0.470-0.725. The combined probability of exclusion and combined discrimination power was 0.9998 and 0.999 999 999 997, respectively.
CONCLUSIONAbove STR loci have high probability of exclusion and discrimination power, which can be used as candidate markers for population genetic research and forensic practice for Tibetans from Lhasa, China.
