A novel human leukocyte antigen-A*33:44 allele revealed by sequence analysis.
- Author:
Haoqiang DING
1
;
Xin YE
;
Yingfeng HUANG
;
Yuan SHAO
;
Yangkai CHEN
;
Wenjie XIA
;
Baiyu XIONG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Alleles; Amino Acid Substitution; Asian Continental Ancestry Group; genetics; Female; HLA-A Antigens; genetics; Humans; Male; Polymorphism, Single Nucleotide; Sequence Analysis, DNA; methods
- From: Chinese Journal of Medical Genetics 2014;31(1):85-87
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the sequence of a novel human leukocyte antigen (HLA)-A*33:44 allele.
METHODSA novel HLA-A allele was found by double-stranded sequencing combined with single-stranded sequencing. The frequency of the novel allele was determined by population survey.
RESULTSGenomic sequence of this novel HLA-A*33:44 allele (accession No. HQ873871) has differed from HLA-B*33:03:01 by one nucleotide in exon 4, which resulted in nt 866 G→ A substitution, which results in an amino acid substitution from Gly(GGT) to Asp(GAT) at codon 265. This alternation is a new single nucleotide polymorphism compared with other HLA-A alleles. The frequency of this new allele is less than 0.0003 in Chinese Han population.
CONCLUSIONA mutation has been found in exon 4 of the novel HLA-A*33:44 allele, which may provide more information for HLA gene study.
