Impact of miR-148a on the proliferation of gastric carcinoma MKN45 cells and its mechanism.
- Author:
Guangting QIU
1
;
Suihan WANG
;
Dapeng LI
;
Jikun LI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Proliferation; Down-Regulation; Humans; Lymphatic Metastasis; MicroRNAs; genetics; Neoplasm Staging; Reverse Transcriptase Polymerase Chain Reaction; Stomach Neoplasms; genetics; Transfection
- From: Chinese Journal of Gastrointestinal Surgery 2014;17(10):1036-1039
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of miR-148a on cell proliferation of human gastric cancer cell lines MKN45.
METHODSExpression level of miR-148a was detected by qRT-PCR in the carcinoma tissues and the tissues adjacent to carcinoma of 60 patients with gastric cancer. Lentivirus packaging was used to establish MKN45 gastric cancer cell line expressing stable miR-148a as transfection group,and the untransfected MKN45 cell line as the control group. Gastric cancer MKN45 cell proliferation was detected by CCK8 method. The target gene of miR-148a was predicted by targetscan. Expression of target gene was examined by Western blotting.
RESULTExpression of miR-148a in gastric cancer tissues of 54 cases(90.0%, 54/60) decreased, and among them, 37 tissue samples(61.7%, 37/60) decreased by 2 times. mir-148a expression in cancer tissues of patients with lymph node metastasis, vascular invasion and TNM stage III(-IIII( was down-regulated more obviously(P<0.05). From the third day after transfection, the growth of MKN45 cells was significantly inhibited(P<0.01). Gene CDC25B might be the target gene of miR-148a according to the results of targetscan. Western blot showed that CDC25B expression in transfection group was lower as compared to control group.
CONCLUSIONMiR-148a expression is down-regulated in gastric cancer tissues and inhibits gastric cancer cell proliferation. CDC25B may be the target gene of miR-148a that plays a role in tumor suppressor.
