Correlation of expression of RhoA (RhoC and their effector ROCK-1 with malignant phenotype of ovarian cancer cells in vitro.
- Author:
Zhi-qiang HAN
1
;
A-li ZHANG
;
Ming-fu WU
;
Yu-lan LIU
;
Gang CHEN
;
Fu-jun LI
;
Qing-lei GAO
;
Guo-ning LIAO
;
Yun-ping LU
;
Shi-xuan WANG
;
Ding MA
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Movement; Female; Gene Expression Regulation, Neoplastic; Humans; Intracellular Signaling Peptides and Proteins; Neoplasm Invasiveness; Neoplasm Metastasis; Ovarian Neoplasms; genetics; metabolism; pathology; Phenotype; Protein Biosynthesis; Protein-Serine-Threonine Kinases; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; Transcription, Genetic; rho GTP-Binding Proteins; biosynthesis; genetics; rho-Associated Kinases; rhoA GTP-Binding Protein; biosynthesis; genetics; rhoC GTP-Binding Protein
- From: Chinese Journal of Oncology 2004;26(7):385-388
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of RhoA, RhoC and their effector ROCK-1 in four ovarian cancer cell lines in vitro and their correlation with invasiveness.
METHODSExpression of RhoA, RhoC and ROCK-1 mRNA and protein in four ovarian cancer cell lines SW626, Skov-3, A2780 and Caov-3 was detected by RT-PCR and Western blot assay. Invasion assay was done in Boyden chamber.
RESULTSThe expression levels of RhoA, RhoC and ROCK-1 mRNA and protein varied in the four different cell lines examined. The expression level of RhoC, but not RhoA and ROCK-1, was significantly correlated with the invasive capability of these cells in vitro (r = 0.95, P < 0.01). Expression of RhoA at the level of transcription was not correlated with that at the translation level. The expression of RhoA and RhoC did not correlate with that of ROCK-1.
CONCLUSIONExpression level of RhoC may serve as an independent parameter in evaluating metastasis and become a new target in inhibiting ovarian cancer metastasis.
