Expressions and activities of Rho GTPases in hypoxia and its relationship with tumor angiogenesis.
- Author:
Yan XUE
1
;
Feng BI
;
Wen-chao LIU
;
Yang-lin PAN
;
Zhe-yi HAN
;
Na LIU
;
Chang-jiang LIU
;
Hui-qin SHEN
;
Xue-yong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Hypoxia; Cell Line, Tumor; Hepatoblastoma; blood supply; metabolism; pathology; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; biosynthesis; genetics; Liver Neoplasms; blood supply; metabolism; pathology; Neovascularization, Pathologic; PTEN Phosphohydrolase; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; Stomach Neoplasms; blood supply; metabolism; pathology; Tumor Suppressor Protein p53; biosynthesis; genetics; Vascular Endothelial Growth Factor A; biosynthesis; genetics; rac1 GTP-Binding Protein; biosynthesis; genetics; rho GTP-Binding Proteins; biosynthesis; genetics
- From: Chinese Journal of Oncology 2004;26(9):517-520
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expressions and activities of Rho GTPases in hypoxia and its relationship with tumor angiogenesis.
METHODSThree tumor cell lines were used in this study: gastric cancer cell lines AGS, SGC7901 and hepatocellular carcinoma cell line HepG2. Expression level of Rac1 mRNA was detected by semi-quantitative RT-PCR. Activity of Rac1 was determined by pull-down assay and expression of HIF-1alpha, VEGF, p53 and PTEN protein was detected by Westernblot.
RESULTSThe expression level of Rac1 mRNA was significantly increased in hypoxia compared to normoxia. Pull-down assay showed that hypoxia-induced activity of Rac1 was elevated in a time-dependent manner and climaxed at 3 hours. The expressions of HIF-1alpha and VEGF protein were up-regulated, while those of PTEN and p53 protein were down-regulated.
CONCLUSIONThese results indicate that hypoxia enhances Rac1 expression which might be involved in tumor angiogenesis by reacting with hypoxia-responsive genes.
