Effect of overexpression of Smad7 gene on cell proliferation.
- Author:
Yan-ying HUO
1
;
Kai-tai ZHANG
;
Bang-yin LI
;
Qin-zhi XU
;
Rui-feng DUAN
;
Ying-chun HU
;
Xiao-qiong XIANG
;
Gang LI
;
De-chang WU
Author Information
- Publication Type:Journal Article
- MeSH: Bronchi; cytology; Cell Proliferation; Cell Transformation, Neoplastic; Cells, Cultured; Cyclin-Dependent Kinase Inhibitor p15; biosynthesis; genetics; Cyclin-Dependent Kinase Inhibitor p21; biosynthesis; genetics; Epithelial Cells; cytology; Humans; Proto-Oncogene Proteins c-myc; biosynthesis; genetics; Signal Transduction; Smad7 Protein; biosynthesis; genetics; Transfection; Transforming Growth Factor beta; biosynthesis; genetics
- From: Chinese Journal of Oncology 2004;26(9):521-524
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of overexpression of Smad7 gene on cell proliferation in human bronchial epithelial cell lines.
METHODSHuman bronchial epithelial cell lines, BEP2D and BERP35T2 cells, were cotransfected with the mammalian expression vectors PCISmad7.neo and pMyc-SEAP, the latter was ac-myc cis-acting enhancer element fused with alkaline phosphatase (SEAP) reporter gene. Expression of c-myc, p15 and p21 mRNA was detected by RT-PCR before and after stable transfection of Smad7 into BEP2D and BERP35T2 cells in order to study the regulation of TGF-beta-mediated growth inhibition.
RESULTSAfter BEP2D and BERP35T2 cells transfected with Smad7, the transcriptional activity of c-myc was significantly increased. Smad7 overexpressing cells showed upregulation of c-myc expression and downregulation of p15 and p21 expression, which contributed to the loss of TGF-beta responses in these cells.
CONCLUSIONOverexpression of Smad7 may facilitate cell proliferation by antagonizing TGF-beta-mediated antiproliferative gene responses.
