EB virus encoded latent membrane protein 1 mediated expression of transcription factor Ets-1 in nasopharyngeal carcinoma cells.
- Author:
Liang ZENG
1
;
Yi-Ping LIU
;
Hai WANG
;
Yong-Guang TAO
;
Xiao-Rong ZHAO
;
Wei LI
;
Ya CAO
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; metabolism; pathology; virology; Cell Line, Tumor; Doxycycline; pharmacology; Herpesvirus 4, Human; Humans; Nasopharyngeal Neoplasms; metabolism; pathology; virology; Phosphorylation; Proto-Oncogene Protein c-ets-1; Proto-Oncogene Proteins; biosynthesis; genetics; Proto-Oncogene Proteins c-ets; RNA, Messenger; biosynthesis; genetics; Transcription Factors; biosynthesis; genetics; Viral Matrix Proteins; biosynthesis; genetics; physiology
- From: Chinese Journal of Oncology 2004;26(8):454-457
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo elucidate the expression of tanscription factor Ets-1 mediated by EB virus encoded latent membrane protein 1 (LMP1) in nasopharyngeal carcinoma (NPC) cells.
METHODSLMP1-expressing NPC HNE2 cells regulated by Tet-on system (pTet-on-LMP1 HNE2) were used. Expression of LMP1 and Ets-1 was observed after induction with Doxycycline (Dox). Expression of Ets-1 mRNA and protein was detected by RT-PCR and Western blot, respectively. The phosphorylation level of Ets-1 protein was examined by co-immunoprecipitation. The DNA binding activity of Ets-1 was detected by electrophoretic-mobility shift assay (EMSA).
RESULTSAfter induction with Dox in pTet-on-LMP1 HNE2 cells, to some extent, the expression of Ets-1 mRNA and protein, its phosphorylation level and DNA binding activity were increased with enhancement of LMP1 expression.
CONCLUSIONLMP1 induces transcriptional activation and expression of Ets-1 which may contribute to the development of NPC.
