Suppression of c-myc expression by interference RNA in HepG2 hepatocellular carcinoma cells.
- Author:
Yang XU
1
;
Yi-Hua WANG
;
Ji-Dong GAO
;
Jue YE
;
Hong-Xia ZHU
;
Ning-Zhi XU
;
Xing-Yu WANG
;
Zong-Tang SUN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Carcinoma, Hepatocellular; metabolism; pathology; Cell Cycle; drug effects; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; drug effects; Genes, myc; Genetic Vectors; Humans; Liver Neoplasms; metabolism; pathology; Proto-Oncogene Proteins c-myc; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; RNA, Small Interfering; genetics; pharmacology; Transfection
- From: Chinese Journal of Oncology 2004;26(8):458-460
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the inhibitory effect of RNA interference (RNAi) on c-myc expression in hepatocellular carcinoma cell line, HepG2.
METHODSExpression vector of c-myc gene-targeting small interference RNA (siRNA) was constructed (psilencer-c-myc) and transfected into HepG2 cells by lipofectamine, and the unloaded vector was used as control (mock). The expression of c-myc mRNA and protein was identified by quantitive PCR and Western blot. Apoptosis of the transfected cells was examined by flow cytometry and immunofluorescent microscopy.
RESULTSAfter HepG2 cells were transfected with psilencer-c-myc, the expression of c-myc mRNA and protein was suppressed with an inhibition rate of 67% compared with the mock-transfected cells. Apoptosis was identified in the transfected HepG2 cells.
CONCLUSIONThe expression of c-myc at transcriptional and translational levels in HepG2 cells transfected with siRNA is markedly inhibited, which may be associated with the induction of apoptosis.
