Expression of endogenous leukemia inhibitory factor in neonatal rats with periventricular leukomalacia.
- Author:
Yu-Ying FAN
1
;
Tao YU
;
Jun-Mei ZHANG
;
Hua WANG
;
Gui-Feng ZHAO
;
Bo LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Disease Models, Animal; Female; Glial Fibrillary Acidic Protein; analysis; Leukemia Inhibitory Factor; analysis; genetics; physiology; Leukomalacia, Periventricular; metabolism; pathology; Male; RNA, Messenger; analysis; Rats; Rats, Wistar
- From: Chinese Journal of Contemporary Pediatrics 2014;16(9):933-938
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the changes of endogenous leukemia inhibitory factor (LIF) in neonatal rats with periventricular leukomalacia (PVL).
METHODSA PVL model of 3-day-old Wistar rats was prepared by left carotid artery ligation followed by 6% oxygen for 4 hours. The rats were sacrificed at 1, 3, 7, 14 and 28 days of hypoxia ischemia (HI), and the brain tissues were sampled. Real-Time PCR and Western blot methods were applied to analyze the expression of LIF mRNA and protein. Double staining immunofluorescence was used to detect the co-expression of LIF and GFAP.
RESULTSAt 1, 3 and 7 days of HI, LIF protein level in the PVL group was higher than in the control group (P<0.01). In the PVL group, the LIF protein level on the third day after HI reached a peak and was higher than the other time points (P<0.01). The change of LIF mRNA expression showed the same tendency with LIF protein. The double staining immunofluorescence showed a co-expression of LIF and GFAP.
CONCLUSIONSLIF mRNA and LIF protein expression in astrocytes show a trend of initial increase followed by steady decline in neonatal rats with PVL, suggesting that endogenous LIF may participate in the repair of PVL.
