Epidemiological survey on the infection of hepatitis E virus among pigs in Henan province.
- Author:
Xiu-ji LI
1
;
Chen-yan ZHAO
;
Jin-ping FAN
;
Ai-jing SONG
;
You-chun WANG
;
Jin-gang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Viral; analysis; immunology; Antigens, Viral; analysis; immunology; China; Genotype; Hepatitis E; epidemiology; immunology; veterinary; virology; Hepatitis E virus; genetics; immunology; isolation & purification; Phylogeny; RNA, Viral; analysis; genetics; Sequence Analysis, DNA; Swine; virology; Swine Diseases; epidemiology; immunology; virology
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(1):24-26
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate hepatitis E virus (HEV) infection among pigs in Henan province.
METHODSA total of 623 swine sera, collected from 5 districts, were divided into two groups, under 3-month of age and over 3-month of age. They were tested for HEV antigen and antibody by using ELISAs, respectively. The sera positive for HEV antigen were tested for HEV RNA with RT-PCR. The positive products of RT-PCR were cloned and sequenced.
RESULTSThe positive rates of anti-HEV antibody of the groups under 3-month and over 3-month of age were 90.27% and 92.55%, respectively, without statistical difference, while those of HEV antigen were 15.93% and 5.69%, respectively, with significant difference. The positive rates of anti-HEV antibody and HEV antigen were significantly different among different districts. HEV RNA was detectable in 5 of 47 HEV antigen positive samples. The sequence analysis showed that in 4 of 5 specimens the sequence belonged to genotype 4 while in the remaining one the sequence was genotype 1.
CONCLUSIONThe prevalence rate of HEV infection in pigs was high in Henan province and the rate differed in different districts.
