Antigenic properties of mutant hepatitis B virus surface antigen.
- Author:
Min-zhuo GUO
1
;
Yao YI
;
Si-yong CHEN
;
Yu BAI
;
Zhi-yuan JIA
;
Sheng-li BI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; COS Cells; Cercopithecus aethiops; Culture Media, Conditioned; metabolism; Enzyme-Linked Immunosorbent Assay; Genetic Vectors; genetics; metabolism; Hepatitis B Surface Antigens; analysis; genetics; immunology; Mutation; Transfection; Viral Envelope Proteins; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(1):48-50
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the antigenic properties of mutant hepatitis B virus surface antigen, to understand the sensitivity of the commercially available HBsAg assays to the variants and to reduce the undetectability of the variants.
METHODSRecombinant eukaryotic expression plasmids for HBsAg. The recombinant eukaryotic expression plasmids pSS1adr, pSS1adw2, pSS1adw2- 145Arg, pSS1adr-126 Asn and pSS1adr-126Ser were transfected into COS-7 cells. HBsAg in the supernatants of transfected cells was detected by using different commercial ELISA kits.
RESULTSThe absorbance value of pSS1adr-126 Asn and pSS1adr-126Ser plasmids were similar to that of the wild type HBsAg, the absorbance value of pSS1adw2-145Arg plasmids was lower than that of the wild type HBsAg.
CONCLUSIONIt is estimated that the antigenicity of HBsAg mainly depended on the amino acid sequence of "a" antigen determinant and its conformation, so 145 amino acid substitutions led to the change of conformation and the antigenicity of variant HBsAg was lower than that of the wild type.
