Study of the subcellular location of the human gene 6 transactivated by nonstructeral protein 5a of hepatitis C virus.
- Author:
Jian-jun WANG
1
;
Ping ZHAO
;
Xue-yuan JIN
;
Jun CHENG
;
Song QING
;
Ning DING
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Genetic Vectors; genetics; metabolism; Hepacivirus; Humans; Intracellular Space; metabolism; Microscopy, Fluorescence; Transcriptional Activation; Transfection; Viral Nonstructural Proteins; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(1):63-64
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo found the subcellular location of the human gene 6 transactivated by nonstructural protein 5A of hepatitis C virus (NS5ATP6).
METHODSGreen fluorescent protein (GFP) expression vector pEGFP- NS5ATP6 was established. The pEGFP- NS5ATP6 was transfected into HepG2 cells, and analyze the subcellular location of the proteins expressed by NS5ATP6 through Green fluorescent microscopy after 24 hours.
RESULTSThe pEGFP- NS5ATP6 gene was successful cloned, NS5ATP6 can express protein in cells and subcellularly located in cell plasma.
CONCLUSIONNS5ATP6 can express protein, and the protein expressed by NS5ATP6 subcellularly located in cell plasma.
