Isolation and identification of Japanese encephalitis virus in Tanghe county, Henan Province
10.3760/cma.j.issn.1003-9279.2008.02.002
- VernacularTitle:河南省唐河县分离到基因1型乙型脑炎病毒
- Author:
Huan-Yu WANG
1
;
Zong-Yu HAO
;
Shi-Hong FU
;
Ai-Mei ZHANC
;
Yu-Xi CAO
;
Fu-Dang SONG
;
Lin-Hong LI
;
Ying HE
;
Huan-Qin WANG
;
Qing TANG
;
Guo-Dong LIANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Encephalitis virus,Japanese;
Genotyping;
Phylogeny
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(2):83-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate Japanese encephalitis virus (JEV) from mosquitoes collected in Tanghe county, Henan province and analyze the genotype of the newly isolated JEV strains and the characteristics of amino acid in the E gone. Methods Viruses were isolated from mosquitoes collected in 2004 and identified by biological, serological and molecular biological methods. PrM and E segments of the newly isolated JEV were amplified by RT-PCR, the PCR products were purified and sequenced. Multiple alignment, phylogenetic and amino acid (AA) analysis were carried out by Clustal X (1.8) program, MEGA 3.1 and GENEDOS (3.2).Results Totally 3722 mosquitoes were collected including Culex, Armigeres, Aedes, Anopheline. Three new JEV strains isolated from Cutex belonged to genotype 1. The homologue of nucleotide and amino acid of E gene between new JEV strains and live attenuated vaccine strain SA14-14-2 was 86.9%-87.7% and 95.2%-97.0%,respectively. Totally there were 12 common sites of amino acid differences in E gene between them. Conclusion Newly isolated viruses in Henan province belonged to JEV genotype 1. It suggests that the vaccine strain SA14-14-2 currently used for preventing JE is able to protect people from JEV infection, although there are some amino acid differences between them.
