Genotype 1 JEV was isolated again from Liaoning Province, China, 2007.
- Author:
Wei-shan MENG
1
;
Ming-hua LI
;
Huan-yu WANG
;
Ji-bo ZHANG
;
Yu GAO
;
Jun DING
;
Shi-hong FU
;
You-gang ZHAI
;
Zhe CHEN
;
Jun-qiao GOU
;
Yu-jun ZHAO
;
Guo-dong LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; Brain; pathology; virology; Cell Line; China; epidemiology; Culicidae; virology; Encephalitis Virus, Japanese; classification; genetics; isolation & purification; Encephalitis, Japanese; epidemiology; virology; Female; Genotype; Insect Vectors; virology; Male; Mice; Molecular Sequence Data; Phylogeny; RNA, Viral; genetics; Sequence Analysis, DNA
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(2):91-94
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate Japanese encephalitis virus (JEV) from mosquitoes collected in Liaoning province and analysis the genotype of new isolated JEV strains and the characters of nucleotide and amino acid in the E gene.
METHODSCollected mosquitoes in Dandong Liaoning Province in August, 2006. Virus isolation was using issue culture cells. Isolated viruses were identified by using serological and molecular methods.
RESULTSTwo new JEV strains, LNDG07-02 and LNDG07-16, were isolated from 1500 mosquitoes were belonging to genotype 1. The identity of nucleotide and amino acid of E gene between new JEV strains and live attenuated vaccine strain SA14-14-2 were 87.8-88% and 97.2%, respectively. Total 11 amino acid sites were differences in E gene between new isolates and SA14-14-2. However, there were no differentiation between the new JEV strains and the isolates in Donggang 2002.
CONCLUSIONGenotype 1 JEV was isolated again from Donggang, since the first isolation of this genotype in 2002. Genotype 1 JEV continues in existence in Donggang Liaoning Province.
