Genotype 1 JEV was isolated again from Liaoning Province China 2007
10.3760/cma.j.issn.1003-9279.2008.02.004
- VernacularTitle:从辽宁省再次分离到基因1型乙型脑炎病毒
- Author:
Wei-Shan MENG
1
;
Ming-Hua LI
;
Huan-Yu WANG
;
Ji-Bo ZHANG
;
Yu GAO
;
Jun DING
;
Shi-Hong FU
;
You-Gang ZHAI
;
Zhe CHEN
;
Jun-Qiao GOU
;
Yu-Jun ZHAO
;
Guo-Dong LIANG
Author Information
1. 沈阳农业大学
- Keywords:
Encephalitis virus,Japanese;
Genotype;
Phylogeny
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(2):91-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate Japanese Encephalitis virus (JEV) from mosquitoes collected in Liaoning province and analysis the genotype of new isolated JEV strains and the characters of nucleotide and amino acid in the E gene. Methods Collected mosquitoes in Dandong Liaoning province in August, 2006. Virus isolation was using issue culture cells. Isolated viruses were identified by using serological and molecular methods. Results Two new JEV strains, LNDG07-02, LNDG07-16, were isolated from 1500 mosquitoes were belonging to genotype 1. The identity of nucleotide and amino acid of E gene between new JEV strains and live attenuated vaccine strain SA14-14-2 were 87.8%-88.0% and 97.2% , respectively. Total 11 amino acid sites were differences in E gene between new isolates and SA14-14-2. However, there were no differentiation between the new JEV strains and the isolates in Donggang 2002. Conclusion Genotype 1 JEV was isolated again from Donggang, since the first isolation of this genotype in 2002. Genotype 1 JEV continues in existence in Donggang Liaoning province.
