Laboratory testing of sepcimens from patients with viral encephalitis from some regions of China
10.3760/cma.j.issn.1003-9279.2008.02.006
- VernacularTitle:我国部分地区病毒性脑炎标本的实验室检测
- Author:
Zi-Qian XU
1
;
Shi-Hong FU
;
Yan-Ping ZHANG
;
Xing-Le LI
;
Xiao-Yan GAO
;
Lei WANG
;
Yu-Xi CAO
;
Li-Hong XU
;
Yu JIN
;
Qing TANG
;
Guo-Dong LIANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Encephalitis,viral;
Noxae
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(2):98-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the viral etiology of viral encephalitis in China by detecting IgM antibody and viral RNA in the clinical samples of patients from some provinces of China by enzyme-linked immunosorbent assay and polymerase chain reaction. Methods Serum and cerebrospinal fluid samples of 771patients with viral encephalitis or meningitis were collected from six provinces of China and were stored at-20℃or - 70℃. Enzyme-linked immunosorbent assays were used for detection of IgM antibody to Japanese encephalitis virus, coxsackievirus, echovirus, herpes simplex virus, measles virus, varicella-zoster virus, mumps virus,cytomegalovirus and Epstein-BaiT virus. Polymerase chain reaction was applied for the detection of viral RNA of enteroviruses and seadornavirus. Results IgM antibody was detected in 567 of 771 (73.5 % ) eases. The most common pathogen was Japanese encephalitis virus (47.0% ,362/771), followed by mumps virus (10.6%, 82/771 ), enteroviruses (8.8% , 68/771 ), herpes simplex virus ( 5.7% , 44/771 ), measles virus ( 0. 4 % , 3/771 ),varicella-zoster virus (0.4% ,3/771 ), Epstein-Barr virus (0.4% , 3/771 ), and cytomegalovirus (0.3 % ,2/771 ) .Enterovirus was positive in 8 cases, seadoruavirus was negative in all the cases by PCR. Conclusion According to the study, Japanese encephalitis was the most important encephalitis in China. Mumps virus was another important pathogen. Enteroviruses and herpes simplex virus were also important pathogens.
