Characterization of the cytopathic efect in human bronchial epithelial cell after human bocavirus infection (HBoV)
10.3760/cma.j.issn.1003-9279.2008.02.009
- VernacularTitle:人博卡病毒感染支气管上皮细胞生物学特征的研究
- Author:
Feng LIN
1
;
Jian-Yi HOU
;
Min-Qiao ZHENG
;
Feng WU
;
Ai-Ping ZENG
;
Hua LI
;
Chang-Hua ZHENG
;
Hong CHEN
;
Xu-Yang LI
;
Gao-Feng RAO
;
Yi-Han MO
;
En-Pei HUANG
Author Information
1. 温州医学院附属温岭医院
- Keywords:
Parvoviridae;
Infection;
Epithelial cells,bronchi;
Cytopathic effect,viral
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(2):107-109
- CountryChina
- Language:Chinese
-
Abstract:
Objective In this study, human bronchial epithelial cell were inoculated with positive sputum specimens of HBoV. Methods After four days infection, cytopathic effect (CPE) were observed by inverted microscopy. These viruses all cause typical cell damages such as rounded and shrivelled, fusion and fallout.Results These damages got quick following increased future degenerations. The other assay result of CPE within the infected cells were observed by inverted microscopy, have typical "owl's eye" plaque and above 90 percent hemadsorption within the infected cells by erthrocytes for hemadsorption technique. The typical fluorescence lump of nucleus within the infected cells was found by indirect immunofluorescence technique. Conclusion Isolation and identification of HBoV could be done in the human bronchial epithelial cell, and we found some characterizing CPE in the human bronchial epithelial cell after HBoV infection. The above studies pave a way for studying pathogenicty of human bocavirus.
