Genome analysis of a newly isolated enterovirus.
- Author:
Jun HOU
1
;
Yan HU
;
Hong-hui SHENG
;
Bing-ke BAI
;
Zhi-jie WANG
;
Pan-yong MAO
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Base Sequence; Cloning, Molecular; Enterovirus; classification; genetics; isolation & purification; Feces; virology; Genome, Viral; genetics; Humans; RNA, Viral; genetics; Sequence Analysis, DNA
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(2):110-112
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo demonstrate molecular characterization of a newly isolated enterovirus.
METHODSVirus were isolated from patient with unknown-causing disease and tested by reverse transcription-polymerase chain reaction (RT-PCR) and 5'3'RACE (rapid amplification of cDNA ends, RACE), in an attempt to obtain the sequence of this newly isolated enterovirus.
RESULTSSequence analysis showed that this newly isolated enterovirus shared 83%-94% nucleotide identity and 91%-100% amino acid identity with enterovirus 89. Phylogenetic analysis indicated that it was probably a new subtype of enterovirus 89.
CONCLUSIONThis newly isolated enterovirus in the stool specimen from patient has the same serotype with enterovirus 89, but it was probably a new subtype of enterovirus 89.
