Study about seroconversion of HBV NAT screening-positive crowd from blood donors
10.3760/cma.j.issn.1003-9279.2008.02.016
- VernacularTitle:无偿献血者乙肝病毒核酸筛查阳性人群的血清转换研究
- Author:
De-Wen WANG
1
;
Tie-Bing WANG
;
Fu-Ping LIU
;
Ling-Ling SHI
Author Information
1. 广东省东莞市中心血站
- Keywords:
Blood donors;
Hepatitis B;
DNA;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(2):127-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the characteristics of seroconversion of HBV NAT screening-positive crowd from blood donors in Dongguan city and provide reference for the safety of blood transfution and disease prevention.Methods With retrospective survey, Nucleic acid testing (NAT) was used to analyze 28 800 HBsAg-negative samples by ELISA from blood donors in Dongguan city from August, 2006 to August, 2007 with Roche Cobas AmpliScreen systems; and follow-up research including NAT for HBV-DNA, ELISA for HBsAg and multiple factors analysis for HBV infection was carried out on HBV NAT screening-positive crowd. Results 10 positive poolings were screened from 28 800 samples; after further detection, 2 of these positive poolings were HBV-DNA negative and 8 HBV-DNA positive samples were found. The 10-week follow-up resesrch on these 8 blood donors showed that6 were HBV-DNA positive and HBsAg-negative at 2 weeks, 4 weeks, 6 weeks, 8 weeks, 10 weeks respectively, 1 wasn't HBsAg positive until 2 weeks and was positive on follow-up, and considered in "window period", 1 was HBV-DNA negative, HBsAg-negative on follow-up. Of these 8,7 were not only migrant labors with poor condition of work, life and health but also in high risk of secondary infection for HBV, in additon they had little idea of therapy or prevention measures of HBV infection and the other 1 was HBV-DNA negative, HBsAg-negative on follow-up, who was in better condition than the above 7 donors. Conclusion NAT is more sensitive than ELISA in screening HBV, but the probability of being false positive of NAT can not be ignored at the same time. On the hand,only screening HBsAg for HBV is relative limitation in high infection region of China. Some factors would have effect on the serum conversion of blood donors including the quality of work and life, therapy or prevention measures.
