The primary application of Direct rapid immunohistochemical test to rabies diagnosis in China
10.3760/cma.j.issn.1003-9279.2008.03.004
- VernacularTitle:直接快速免疫组化法在我国狂犬病诊断中的初步应用
- Author:
Xiao-Yan TAO
1
;
Niezgoda MICHAEL
;
Jia-Liang DU
;
Hao LI
;
Xiao-Guang WANG
;
Ying HUANG
;
Yang JIAO
;
Lei CAO
;
Qing TANG
;
Guo-Dong LIANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Rabies;
Diagnosis;
differential;
Immunohistochemistry
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(3):168-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective Evaluation of the direct rapid immumohisto-chemical test (DRIT) for laboratory surveillance of rabies. Methods 72 brain specimens of domestic dogs or patients collected from Guizhou, Guangxi, Hunan, Anhui, Jiangsu and Yunnan provinces were detected by conventional Methods including Direct Fluorescent-antibody Assay (DFA) and Reverse Transcription Polymerase Chain Reaction (RT-PCR), and by DRIT which was newly developed in the Rabies Section of the Centers for Disease Control and Prevention in the United States. The sensitivity and specificity of DRIT were evaluated by compare of the three Results . By analysis of the index including cost of experiment, technique requirement and so on, the advancement and applicability of DRIT were discussed. Results Compared with DFA and RT-PCR, DRIT will be more applicable for laboratories with limited funds and weak techniques because of its lower cost needed and simpler techniques required while its sensitivity and specificity are equal to the other two Methods . Conclusion DRIT is more valuable in rabies diagnosis and more applicable for extension and popularization in rabies laboratory surveillance in local CDC.
