Hantavirus mucosal vaccine through different mucosal with heat-labile enterotoxin B subunit as adjuvants.
- Author:
Shou-Chun CAO
1
;
Jian-Dong LI
;
Peng LU
;
Feng LIU
;
Qin WANG
;
Qin-Zhi LIU
;
Quan-Fu ZHANG
;
Chuan LI
;
Qi AN
;
Mi-Fang LIANG
;
De-Xin LI
Author Information
- Publication Type:Journal Article
- MeSH: Adjuvants, Immunologic; administration & dosage; genetics; Animals; Antibodies, Viral; blood; Bacterial Toxins; administration & dosage; genetics; immunology; Enterotoxins; administration & dosage; genetics; immunology; Escherichia coli Proteins; administration & dosage; genetics; immunology; Female; Hantavirus; genetics; immunology; Hantavirus Infections; immunology; virology; Humans; Immunity, Mucosal; Immunoglobulin A; blood; Immunoglobulin G; blood; Mice; Mice, Inbred C57BL; Random Allocation; Viral Vaccines; administration & dosage; genetics; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(3):174-176
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluation the effect of different mucosal vaccination pathway on hantavirus with the recombinant E. coli heat-labile enterotoxin B subunit (rLTB) as adjuvant.
METHODSThe rLTB was expressed and purified. Take the inactivated hantavirus strain 84Fli as vaccine, and immunized C57 BL/6 mice through intranasal, oral and vaginal respectively. Specific IgG and sectory IgA were detected by ELISA in serum, and vaginal washing samples respectively.
RESULTSThe rLTB was efficiently expressed under the induction of lactose, identified by western blotting and GM-1 binding experiment. The vaccination through intranasal, oral and vaginal, can induce IgG and sectory IgA response.
CONCLUSIONInactivated hantavirus can produce mucosal immune response with rLTB as adjuvants through intranasal, oral and vaginal vaccination respectively.
