Hantavirus mucosal vaccine through different mucosal with heat-labile enter toxin B subunit as adjuvants
10.3760/cma.j.issn.1003-9279.2008.03.006
- VernacularTitle:汉坦病毒经不同途径黏膜免疫效果研究
- Author:
Shou-Chun CAO
1
;
Jian-Dong LI
;
Peng LU
;
Feng LIU
;
Qin WANG
;
Qin-Zhi LIU
;
Quan-Fu ZHANG
;
Chuan LI
;
Qi AN
;
Mi-Fang LIANG
;
De-Xin LI
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Hantan virus;
Immunity,mucosal;
Vaccination
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(3):174-176
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluation the effect of different mucosal vaccination pathway on hantavirus with the recombinant E. coli heat-labile enterotoxin B subunit (rLTB) as adjuvant. Methods The rLTB was expressed and purified. Take the inactivated hantavirus strain 84F1i as vaccine, and immunized C57 BL/6 mice through intranasal, oral and vaginal respectively. Specific IgG and sectory IgA were detected by ELISA in serum, and vaginal washing samples respectively. Results The rLTB was efficiently expressed under the induction of Lactose, identified by western blotting and GM-1 binding experiment. The vaccination through intranasal, oral and vaginal, can induce IgG and sectory lgA response. Conclusion Inactivated hantavirus can produce mucosal immune response with rLTB as adjuvants through intranasal, oral and vaginal vaccination respectively.
