Recombinant envelope glycoprotein domain Ⅲ of dengue virus inhibit virus infection
10.3760/cma.j.issn.1003-9279.2008.03.007
- VernacularTitle:重组登革病毒E蛋白结构域Ⅲ抑制登革病毒感染的初步研究
- Author:
Peng LU
1
;
Yan WEI
;
Shou-Chun CAO
;
Jian-Dong LI
;
Qin-Zhi LIU
;
Quan-Fu ZHANG
;
Chuan LI
;
Fang MIAO
;
Shuo ZHANG
;
Xiao-Tong HANG
;
MI-Fang LIANG
;
De-Xin LI
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Dengue virus;
Viral structural proteins
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(3):177-179
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the ability of dengue virus recombinant envelope protein domain expressed in E. coil to inhibit virus infection and induce the neutralizing antibody. Methods E Ⅲ protein of Dengue virus serotypes 1-4 were expressed in E. coli BL21 (DE3) then purified. Recombinant proteins were tested to inhibit DV2 from infecting BHK-21 cell. Rabbits were immunized with recombinant proteins to produce anti-E Ⅲserum. Antibody titers were determined by neutralizing assay. Results The recombinant E Ⅲ proteins of Dengue virus serotypes 1-4 were expressed in E. coli. They effectively protected BHK cells in culture against DV2infection. All four type anti-E Ⅲ sera can neutralize DV2 but their efficacies are different. Conclusion E Ⅲproteins of dengue virus expressed in E. coli can directly inhibit DV2 infection. Neutralizing antibodies were induced by E Ⅲ proteins. Both E Ⅲ protein of dengue virus and the neutralizing antibodies they induced are more efficient in inhibiting homologous dengue serotypes infection than heterologous serotypes.
