The differential expression of the human lung carcinoma cells infected with high pathogenic avian influenza virus A/Anhui/1/2005 (H5N1).
- Author:
Xiao-Fen HU
1
;
Qin-Zhi LIU
;
Chuan LI
;
Jie DONG
;
Jian-Fang ZHOU
;
Min WANG
;
Yue-Long SHU
;
Hong-Tu LIU
;
Mi-Fang LIANG
;
De-Xin LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Cell Line, Tumor; Gene Expression Profiling; Humans; Influenza A Virus, H1N1 Subtype; physiology; Influenza A Virus, H5N1 Subtype; physiology; Influenza, Human; genetics; metabolism; virology; Oligonucleotide Array Sequence Analysis
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(3):180-182
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify genes in human cells infected with high pathogenic avian influenza viruses H5N1.
METHODSThe lung carcinoma cells line A549 was infected with H5N1 and H1N1, respectively. We harvested the infected cells at the different time points after infection and screened the genes with differential expression via microarray technology. The candidate genes were selected and confirmed by quantitative real-time PCR.
RESULTSThe spectrum of genes with the differential expression in the cells infected with H5N1 was obtained and 16 candidate genes were identified in the cellular apoptosis pathway, mTOR pathway, and the cellular immunity as well.
CONCLUSIONSOur results suggest that H5N1 exert a stronger impact on eliciting apoptosis of infected cells than the common influenza virus H1N1.
