The differential expression of the human lung carcinoma cells infected with high pathogenic avian influenza virus A/Anhui/1/2005(HSN1)

VernacularTitle:高致病性禽流感病毒H5N1安徽株A/Anhui/1/2005感染人肺癌上皮细胞(A549)的差异表达分析
Author: Xiao-Fen HU ¹ ; Qin-Zhi LIU ; Chuan LI ; Jie DONG ; Jian-Fang ZHOU ; Min WANG ; Yue-Long SHU ; Hong-Tu LIU ; Mi-Fang LIANG ; De-Xin LI
Author Information

1. 中国疾病预防控制中心病毒病预防控制所
Keywords: Orthomyxo viridae; Influenza A virus,avian; Expressed sequence tags; Chip; Polymerase chain reaction
From: Chinese Journal of Experimental and Clinical Virology 2008;22(3):180-182
CountryChina
Language:Chinese
Abstract: Objective To identify genes in human cells infected with high pathogenic avian influenza viruses H5N1. Methods The lung carcinoma cells line A549 was infected with H5N1 and H1N1, respectively. We harvested the infected cells at the different time points after infection and screened the genes with differential expression via micro array technology. The candidate genes were selected and confirmed by quantitative real-time PCR. Results The spectrum of genes with the differential expression in the cells infected with H5N1 was obtained and 16 candidate genes were identified in the cellular apoptosis pathway, mTOR pathway, and the cellular immunity as well. Conclusion s Our Results suggest that H5N1 exert a stronger impact on eliciting apoptosis of infected cells than the common influenza virus H1N1.