Influence of avian influenza virus NS1 protein on the expression of IP-10 in BEAS-2B cells

VernacularTitle:H5N1禽流感病毒NS1蛋白与干扰素诱导蛋白10表达的相关性研究
Author: Xiao-Jun JIA ¹ ; Jian-Fang ZHOU ; Jing-Yu WANG ; Jie DONG ; Hong BO ; Zi LI ; Kui-Biao LI ; Yu LAN ; Yue-Long SHU
Author Information

1. 西北农林科技大学
Keywords: Influenza A virus,avian; Genes,NS1; Genes,IP-10; Cells,cultured
From: Chinese Journal of Experimental and Clinical Virology 2008;22(3):183-185
CountryChina
Language:Chinese
Abstract: Objective To investigate the influence of avian influenza virus (AIV) NS1 protein on the expression of interferon-inducible protein 10 (IP-10). Methods NS1 gene from virus A/Anhui/1/2005 (H5N1),NS1 gene inserted with 80-84 amino acids from virus A/Anhui/1/2005(H5N1)and NS1 gene from virus A/Puerto Rico/8/1934 (H1N1) were cloned into the eukaryotic expression vector pEGFP-N1, and transected into BEAS-2Bcells, IP-10 expression level in transected cells was detected by flow cytometry. Results Compared with the control group pEGFP-N1, Expression of these three different NS1 genes can down-regulate the expression of IP-10in BEAS-2B cells, but there is no significant difference as to the lower level among them. Conclusion NS1protein of A/Anhui/1/2005(H5N1) can down-regulate the expression level of IP-10, but this may not clarify its relationship with the virulence of AIV.