Construction of recombinant vaccinia virus expressing HPV18E7E6 fusion proteins and detection of its immunogenicity in mice
10.3760/cma.j.issn.1003-9279.2008.03.011
- VernacularTitle:表达HPV18E7E6的重组痘苗病毒构建及免疫效果的初步研究
- Author:
Li ZHAO
1
;
Jiao REN
;
Jing FENG
;
Jian GAO
;
Hui ZHANG
;
Hou-Wen TIAN
;
Li RUAN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Papillomavirus,human;
Cervix neoplasms;
Vaccines;
Vaccinia virus
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(3):189-191
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct one recombinant vaccinia virus expressing the HPV18.E6 and E7fusion proteins as HPV18 therapeutic vaccine candidate, and test its immunogenicity. Methods The fusion E7E6genes were synthesized and mutated to inactivate their oncogenic potential, and inserted into a vaccine virus plasmid vector to construct one recombinant vaccinia virus. Finally its immunogenicity was characterized in immunized mice. Results One recombinant vaccinia virus expressing HPV18 E7E6 fusion proteins was constructed. Sequencing Results of PCR products and Western blot tests showed that the E7E6 fusion genes were correct and expressed in CEF cells infected with the recombinant vaccinia virus. The specific antibodies against E6and E7 proteins were elicited, however no positive responses were detected by ELISPOT in immunized mice. Conclusion s One recombinant vaccinia virus expressing HPVI8 E7E6 fusion proteins was generated and elicited specific antibodies against E6 and E7 proteins, but detected no positive cellular immune responses in immunized mice, which will provide the basis to develop the different animal model for examining the cellular immune responses of HPV18E6 and E7 proteins.
