CD8+ cell noncytotoxic antiviral response (CNAR) to HIV in nosocomial HIV-infected individuals.
- Author:
Lin YUAN
1
;
Li-Ying MA
;
Xi-Hui ZANG
;
Xiang-Dong MENG
;
Hong PENG
;
Quan-Bi ZHAO
;
Hui XING
;
Yi-Ming SHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged, 80 and over; Anti-HIV Agents; therapeutic use; CD4-Positive T-Lymphocytes; drug effects; immunology; CD8-Positive T-Lymphocytes; drug effects; immunology; Cells, Cultured; Cross Infection; drug therapy; immunology; Female; HIV; drug effects; immunology; HIV Infections; drug therapy; immunology; Humans; Male; Middle Aged
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(3):195-197
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the CD8+ cell noncytotoxic antiviral response (CNAR) to HIV in nosocomial HIV infected individuals, and reveal the relationship between the CNAR and CD4+ cell count.
METHODCD8+ cells from HIV-1 sero-positive individuals were separated by immunomagnetic beads and mixed with CD4+ cells at different CD8 CD4 cell input ratios (2:1, 1:1, 0.5:1 and 0.25:1). Reverse transcriptase (RT) activity of cocultured supernatant was tested and compared with negative control and the suppression rate of HIV-1 replication was measured.
RESULTThe average CD8:CD4 cell input ratios to reach 80% suppression of HIV replication in the group with CD4 < 300/microl and CD4 > 300/microl were 2.4:1 and 1.3:1, respectively (P < 0.05).
CONCLUSIONCNAR activity in HIV infected individuals is associated with CD4+ cell count. The ability to suppress HIV replication in subjects with CD4 > 300 is stronger than those with CD4 < 300.
