CD8+ cell noncytotoxic antiviral response (CNAR) to HIV in nosocomial HIV-infected individuals

VernacularTitle:医源性HIV感染者CD8+细胞非细胞毒性HIV抑制作用的研究
Author: Lin YUAN ¹ ; Li-Ying MA ; Xi-Hui ZANG ; Xiang-Dong MENG ; Hong PENG ; Quan-Bi ZHAO ; Hui XING ; Yi-Ming SHAO
Author Information

1. 中国疾病预防控制中心性病艾滋病预防控制中心
Keywords: Antigens,CD8; T-lymphocytes suppressor-inducer; CD4-CD8 Ratio; HIV infections
From: Chinese Journal of Experimental and Clinical Virology 2008;22(3):195-197
CountryChina
Language:Chinese
Abstract: Objective To study the CD8+ cell noncytotoxic antiviral response (CNAR) to HIV in nosocomial HIV infected individuals, and reveal the relationship between the CNAR and CD4 cell count. MethodCD8+ cells from HIV-1 sero-positive individuals were separated by immunomagnetic beads and mixed withCD4+ cells at different CD8 CD4 cell input ratios (2:1,1:1,0.5:1 and 0.25:1). Reverse transcriptase (RT) activity of cocultured supernatant was tested and compared with negative control and the suppression rate of HIV-1 replication was measured. Result The average CD8 : CD4 cell input ratios to reach 80% suppression of HIV replication in the group with CD4<300/μl and CD4>300/μl were 2.4:1 and 1.3:1, respectively (P<0.05).Conclusion CNAR activity in HIV infected individuals is associated with CD4<'+> cell count. The ability to suppress HIV replication in subjects with CD4>300 is stronger than those with CD4<300.