Effects of Newcastle disease virus on the mitochondria of human gastric carcinoma BGC-823 cells.
- Author:
Kai-Yang LIU
1
;
Jian-Guo QU
;
Jin-Jun LIU
;
Fang LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma; enzymology; metabolism; virology; Cell Line, Tumor; Chick Embryo; Cytochromes c; metabolism; Humans; Membrane Potential, Mitochondrial; Mitochondria; enzymology; metabolism; virology; Newcastle Disease; enzymology; metabolism; virology; Newcastle disease virus; physiology; Sodium-Potassium-Exchanging ATPase; metabolism; Stomach Neoplasms; enzymology; metabolism; virology
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(3):198-200
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore changes in structure and function of the mitochondria of human gastric carcinoma BGC-823 cells after Newcastle disease virus (NDV) infection.
METHODSElectron microscopy was applied to observe the structure of mitochondria; Rhodamine 123 staining was used to determine the mitochondrial membrane potential; the activity of Na(+)-K(+)-ATPase and Ca(2+)-ATPase were also determined and the release of cytochrome C was detected by Western blotting.
RESULTSThe structure of mitochondria in the tumor cells infected with NDV changed distinctly. In the infected group the activity of mitochondrial Na(+)-K(+)-ATPase and Ca(2+)-ATPase significantly declined (P < 0.01), and compared with control cells, mitochondrial trans-membrane potential was decreased. NDV infection induced the decrease of cytochrome C levels.
CONCLUSIONThe effects of NDV infection on the structure and functions of mitochondria of human gastric carcinoma BGC-823 cells might play a role in the oncolysis of NDV.
