Effects of Newcastle disease virus on the mitochondria of human gastric carcinoma BGC-823 cells

VernacularTitle:新城疫病毒对BGC-823胃癌细胞线粒体的影响
Author: Kai-Yang LIU ¹ ; Jian-Guo QU ; Jin-Jun LIU ; Fang LIU
Author Information

1. 河北北方学院
Keywords: Newcastle disease virus; Stomach neoplasms; Adenocareinoma; Ceils,cultured; Mitochondria; Adenosinetriphosphatase
From: Chinese Journal of Experimental and Clinical Virology 2008;22(3):198-200
CountryChina
Language:Chinese
Abstract: Objective To explore changes in structure and function of the mitochondria of human gastric carcinoma BGC-823 cells after Newcastle disease virus (NDV) infection. Methods Electron microscopy was applied to observe the structure of mitochondria; Rhodamine 123 staining was used to determine the mitochondrial membrane potential; the activity of Na+-K+-ATPase and Ca2+-ATPase were also determined and the release of cytochrome C was detected by Western blotting. Results The structure of mitochondria in the tumor cells infected with NDV changed distinctly. In the infected group the activity of mitochondrial Na+-K+-ATPase and Ca2+-ATPase significantly declined (P<0.01), and compared with control cells, mitochondrial trans-membrane potential was decreased. NDV infection induced the decrease of cytochrome C levels. Conclusion The effects of NDV infection on the structure and functions of mitochondria of human gastric carcinoma BGC-823 ceils might playa role in the oncolysis of NDV.