Immunosuppression induced by Measles virus in adult patients is not related to CD4+ CD25+ regulatory T cell induction
10.3760/cma.j.issn.1003-9279.2008.03.018
- VernacularTitle:成人麻疹细胞免疫与CD4+ CD25+调节性T细胞的关系
- Author:
Mei-Zhong LI
1
;
Fu-De XU
;
Xue-Huan HUANG
;
Xin-Chun CHEN
;
Qi-Wen DENG
;
Shui-Teng LIU
;
Yan LIU
;
Liu-Mei XU
;
Huo-Sheng WANG
;
Jian-Jun CUI
Author Information
1. 广东省深圳市东湖医院
- Keywords:
Measles virus;
Immunosuppression;
CD4 Lymphocyte count;
CD25 Lymphocyte counts
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(3):211-213
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate of the relationship of the immunosuppression induced by Measles virus in adult patients and CD4+ CD25+ regulatory T cell. Methods Thirty-four patients with measles and 27healthy control subjects were included in this study. The whole blood was collected and CD4+ CD25+ cell andFoxP33 cell were analyzed by flow cytometry, and CD4+ CD25- and CD4+CD25+ T lymphocytes were isolated from PBMCs of patients with measles or healthy donors, CD4+ CD25- T cells were cultured in absence or presence of anti-CD3, or BCG, or live attenuated MV. The cell culture supernatant was collected after 72 hours and the concentration of IFN-γ and IL-10 was determined. Results Compared to healthy donors, we observed a reduction of the number of white blood cells and lymphocytes in patients with measles, but there was not significantly different in the frequency of CD4+ CD25+ T cells and CD4+ CD25high T cells with in the total CD4+ population in the blood. Treg from both measles patients and healthy controls significantly inhibited IFN-γ production by CD4+ CD25- T cells in response to anti-CD3 stimulation. Conclusion Induction and expansion of Treg may not represent a mechanism involved in the establishment of immune suppression by MV.
