Detection of HBV DNA among HBsAg negative children after prevention for mother-to-infant transmission.
- Author:
Shuang ZHANG
;
Tao BIAN
;
Feng WANG
;
Li-Ping SHEN
;
Hong-Xia YAN
;
Yong ZHANG
- Publication Type:Journal Article
- MeSH: Child; Child, Preschool; DNA, Viral; blood; genetics; Female; Hepatitis B; blood; prevention & control; transmission; Hepatitis B Surface Antigens; blood; genetics; Hepatitis B virus; genetics; Humans; Infectious Disease Transmission, Vertical; prevention & control; Male; Mutation
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(3):214-215
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEDetection HBV DNA among HBsAg negative children who have been vaccined at birth, in order to improve the evaluation of the indicator for HBV DNA infection.
METHODSSelection HBsAg negative children who have been vaccined at birth and then detection HBV DNA from sera using QIAamp Viral DNA Mini Kit, HBV DNA s region was obtained by nested PCR and sequencing.
RESULTS12 of the 140 children were HBV DNA detected were positive and the infectious rate was 8.6% . No mutant of the 12 HBV DNA in "a" determinant.
CONCLUSIONTo evaluate the effection of the prevention of HBV mother-to-child transmission, the standard method should be established. The detection of HBV DNA should be included in the future.