Quantitative detection of hepatitis B virus covalently closed circular DNA in sera of chronic hepatitis B patients with a newly established assay.
- Author:
Yan-Wei ZHONG
1
;
Zhao-Ling LIANG
;
Xiao-Qiang REN
;
Xiao-Dong LI
;
Zhi-Hui XU
;
Le LI
;
Dong-Ping XU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; DNA Primers; genetics; DNA, Circular; blood; genetics; DNA, Viral; blood; genetics; Female; Hepatitis B virus; genetics; Hepatitis B, Chronic; virology; Humans; Male; Middle Aged; Polymerase Chain Reaction; methods; Young Adult
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(3):225-227
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo quantitatively detect hepatitis B virus covalently closed circular DNA (HBV cccDNA) in sera of chronic hepatitis B patients with a newly established assay.
METHODSPrimers and probe were designed in highly conservative region of HBV DNA. DNA was extracted from 175 sera samples of chronic hepatitis B patients, and was treated with plasmid-Safe-ATP-dependent Dnase(PSAD) to eliminate the relaxed circular DNA (rcDNA). The products were amplified by real-time PCR with primers spanning.
RESULTSThe detection rate of serum HBV cccDNA was found to correlate directly with serum HBV DNA loading. HBeAg positive chronic hepatitis B patients had higher serum HBV cccDNA levels than HBeAg negative chronic hepatitis B patients.
CONCLUSIONThe method is good because of the high specificity. It can be used for detection of HBV cccDNA. DNA;
