Analyses of the expressions of GFAP in the brain tissues of hamsters infected with various amounts of scrapie strain 263K at terminal stage

VernacularTitle:不同剂量羊瘙痒因子263K颅内感染仓鼠临床终末期脑中GFAP表达的研究
Author: Chan TIAN ¹ ; Bao-Yun ZHANG ; Qi SHI ; Jun HAN ; Chen GAO ; Lu HAN ; Xiao-Ping DONG
Author Information

1. 中国疾病预防控制中心病毒病预防控制所
Keywords: Prions; Scrapie agent 263K; Fibrin
From: Chinese Journal of Experimental and Clinical Virology 2008;22(4):241-243
CountryChina
Language:Chinese
Abstract: Objective To investigate whether gliosis in the brain tissues of the hamsters infected with various amounts of scrapie strain 263K is correlated with the inoculation doses or the incubation times. Methods The total values of glial fibrillary acidic protein (GFAP) in brains were evaluated by Western Blots and the GFAP-stained cells were detected by immunohistochemistry (IHC). The characteristics of GFAP distributions among various groups were defined by quantitive and statistic analyses. Results Compared with the brain tissues of normal hamsters, remarkably higher total GFAP levels and more GFAP-stained cells were observed in the brain tissues of infected ones, howbeit, no significant difference was addressed among the infected groups. Conclusion Inoculations of various amounts of scrapie strain 263K into experimental hamsters intracerebrally induced the similar patterns of gliosis in the brains at the clinically terminal stage, regardless of infectious doses and incubation times.