Construction of helper plasmids used in reverse genetic system of a cell adapted rabies virus strain
10.3760/cma.j.issn.1003-9279.2008.04.003
- VernacularTitle:细胞适应株狂犬病病毒反向遗传系统辅助质粒的构建
- Author:
Ying HUANG
1
;
Rong-Liang HU
;
Qing TANG
Author Information
1. 吉林大学
- Keywords:
Rabies virus;
Plasmids;
Viral structural proteins
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(4):247-250
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct four helper plasmids which are necessary in reverse genetic system of a cell adapted rabies virus strain. Methods Nucleoprotein gene, phosphprotein gene, glycoprotein gene and the viral RNA polymerse gene are amplified by RT-PCR. The four gene fragments were cloned into an expression vector respectively after sequencing to construct four helper plasmids. Results The four structral genus were amplified successfully, the sequence were correct. And all the structral protein gene fragments were cloned into expression vectors, the results were correct identified by cleavage reaction. Conclusion Construction of the four helper plasmids have been done successfully, and it is the basis for rescuing the adapted cell rabies virus strain from cloned cDNA.
