Detection of human papillomavirus in tissues of esophageal carcinomas by polymerase chain reaction

VernacularTitle:应用聚合酶链反应检测食管癌组织中人乳头瘤病毒
Author: Shu-Ying LI ¹ ; Ying LI ; Li-Dong WANG ; Xiao-Zhou WU ; Ling ZHOU ; Xiao-Yu ZHAO ; Hong-Tu LIU ; Yi ZENG
Author Information

1. 华北煤炭医学院
Keywords: Esophageal neoplasms; Carcinoma; Papillomavirus,human; Polymerase chain reaction
From: Chinese Journal of Experimental and Clinical Virology 2008;22(4):251-253
CountryChina
Language:Chinese
Abstract: Objective To investigate the relationship between the human papillomavirus (HPV) and esophageal carcinomas. Methods We detected HPV DNA in 31 fresh tissue of esophageal carcinomas from Linzhou City, Henan Province, by PCR with the general primer set of GP5+/6+ for HPV L1 gene and type-specific primer sets for HPV16 and 18 as well. Results 29 from 31 esophageal carcinoma samples were HPV positive and the rate was 93.5%. Among the samples detected, 19 were HPV16E6 positive and rate was 61.3%,eight were HPV18 E6 positive and rate was 25.8% ; our result also showed five were the multiple infection containing HPV16 and 18 as well and the rate was 71.0%. Conclusion The high rate of HPV in the esophageal carcinoma samples suggested that HPV plays an etiologic role in the development of esophageal cancer in Linzhou City, Henan Province.