Dendritic cells transfected with recombinant adenovirus Ad5F35-LMP2 induces LMP2 specific immunity mediated by cytotoxic T lymphocytes in vitro
10.3760/cma.j.issn.1003-9279.2008.04.005
- VernacularTitle:Ad5F35-LMP2重组腺病毒修饰DC体外诱导特异性T细胞免疫的研究
- Author:
Wu-Ning MO
1
;
Ling ZHOU
;
Zhan WANG
;
An-Zhou TANG
;
Guang-Wu HUANG
;
Yi ZENG
Author Information
1. 广西医科大学附属第一医院
- Keywords:
Adenoviridae;
Dendritic cells;
Herpesvirus 4,human;
Membrane proteins;
T lymphocytes
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(4):254-256
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe whether dendritic cells(DCs) transfected with recombinant adenovirus AdSF35-LMP2 induces LMP2 specific immunity mediated by cytotoxic T lymphocytes in vitro. Methods Dendritic cells have been generated in vitro, and cocultured with autologous T cell after the DCs were infected with Ad5F35-LMP2, then the proliferation of the induced T cells and their cytotoxic activity against CNE-2 tumor cells which express EBV-LMP2 protein on membrane were assessed by MTT method. Results The dendritic cells could be transfected with Ad5F35-LMP2 and the CTL activated by Ad5F35-LMP2-DC could effectively suppress the proliferation of CNE-2 cells compared with control groups. Conclusion The dendritic cells transfected with recombinant adenovirus Ad5F35-LMP2 showed cytotoxicity effect by activating T lymphocytes.
