Intracellular clearance of Coxsakievirus B3 infection by short interfering RNA and its mechanism study.
- Author:
Zong-Hui XIAO
1
;
Ji-Sheng HAN
;
Hai-Lan YAO
;
Zhe-Wei LIU
Author Information
- Publication Type:Journal Article
- MeSH: Coxsackievirus Infections; therapy; virology; Enterovirus B, Human; genetics; metabolism; HeLa Cells; Humans; RNA Interference; RNA, Small Interfering; genetics; therapeutic use; RNA, Viral; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(4):260-262
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the possibility of short interfering RNA (siRNA) inhibiting Coxsackievirus B3 (CVB3) infection in vitro, and discover the mechanism initially.
METHODSWe obtained proper effective dosage of siRNA by observing cytopathic effect (CPE). Estimate its antiviral activities and its pathway of siRNA by Western Blot assay and RT-PCR.
RESULTSResults showed that siRNA-3753 can be effectively transfected into HeLa cells, we can achieve a high transfection efficiency up to 98.77% and its effect can last for 48 h stably in cells. 0.6 micromol/L siRNA-3753 got a high inhibiting effect of virus and didn't show any toxicity to cells. So we consider this concentration as the experimental concentration. siRNA-3753 can debase virus reproduction. The antiviral effect is sequence-specific and is not attributable to either interferon or the interferon response effectors protein kinase R (PKR).
CONCLUSIONThe data confirmed that siRNA can effectively inhibit CVB3 infection in vitro, its antivirus effect was gained from specific debase of virus genome.
