Intracellular clearance of Coxsakievirus B3 infection by short Interfering RNA and its mechanism study

VernacularTitle:短双链RNA对CVB3病毒细胞内清除作用的可行性研究及其机制探讨
Author: Zong-Hui XIAO ¹ ; Ji-Sheng HAN ; Hai-Lan YAO ; Zhe-Wei LIU
Author Information

1. 首都儿科研究所
Keywords: RNA; Coxsackievirns infectious
From: Chinese Journal of Experimental and Clinical Virology 2008;22(4):260-262
CountryChina
Language:Chinese
Abstract: Objective To evaluate the possibility of short interfering RNA (siRNA) inhibiting Coxsackievirns B3 (CVB3) infection in vitro, and discover the mechanism initially. Methods We obtained proper effective dosage of siRNA by observing cytopathic effect (CPE). Estimate its antiviral activities and its pathway of siRNA by Western Blot assay and RT-PCR. Results Results showed that siRNA-3753 can be effectively transfected into HeLa cells, we can achieve a high transfection efficiency up to 98.77 % and its effect can last for 48 h stably in cells. 0.6 μmol/L siRNA-3753 got a high inhibiting effect of virus and didn't show any toxicity to cells. So we consider this concentration as the experimental concentration, siRNA-3753 can debase virus reproduction. The antiviral effect is sequence-specific and is not attributable to either interferon or the interferon response effectors protein kinase R (PKR). Conclusion The data confirmed that siRNA can effectively inhibit CVB3 infection in vitro, its antivirus effect was gained from specific debase of virus genome.