The correlation between the polyomavirus DNA load and the dose of immunosuppressant.
- Author:
Peng CAI
1
;
Zhi-Ping TENG
;
Tong WU
;
Jian-Feng ZHANG
;
Ying LIU
;
Zheng WANG
;
Wan-Xia ZHENG
;
Guo-Lin XU
;
Chun-Rong TONG
Author Information
- Publication Type:Journal Article
- MeSH: Bone Marrow Transplantation; adverse effects; Cyclosporine; adverse effects; blood; Cystitis; prevention & control; virology; DNA, Viral; genetics; urine; Dose-Response Relationship, Drug; Humans; Immunosuppressive Agents; adverse effects; blood; Polyomavirus; genetics; isolation & purification; Polyomavirus Infections; prevention & control; virology; Transplantation, Homologous; adverse effects; Viral Load
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(4):272-274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the correlation between the polyomavirus DNA load and the dose of immunosuppressant in patients with allogene bone marrow transplantation (allo-BMT) for preventing the development of post-transplantational hemorrhagic cystitis.
METHODSSerial blood and urine samples from 122 cases of allo-BMT recipients were obtained and DNA was extracted from urine samples. Polyomavirus DNA-specific probe was synthesized and Fluorescence quantitative polymerase chain reaction was used for detecting the polyomavirus DNA loads and Fluorescence polarization immunoassay (FPIA) was performed for determining the dose of immunosuppressant Cyclosporin A (CsA) in blood.
RESULTSThe altered polyomavirus DNA load in urine was followed by concentration of CsA in blood. When the concentration of CsA in blood was higher than 86-105 ng/ml, the positive rate of polyomavirus DNA load was significantly increased and both presented the linable correlation.
CONCLUSIONIn immunosuppression condition, polyomavirus DNA load correlated to the dose of immunosuppressant, which increased the risk of post-transplantational hemorrhagic cystitis.
