The immunostimulatory study on peripheral blood mononuclear cell and CD4+ T lymphocyte of HBV infections that were activated by CpG oligonucleotide.
- Author:
Tian-Xin XIANG
1
;
Xiao-Ping WU
;
Ming-Hui LI
;
Yao XIE
;
Jia-Gan HUANG
;
Xiao-Yan MA
;
Xiao-Quan LI
;
Bin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; CD4-Positive T-Lymphocytes; immunology; Cells, Cultured; Female; Hepatitis B Antigens; immunology; Hepatitis B virus; immunology; Hepatitis B, Chronic; immunology; virology; Humans; Interferon-gamma; immunology; Leukocytes, Mononuclear; immunology; Male; Middle Aged; Oligodeoxyribonucleotides; immunology; Young Adult
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(4):278-280
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of CpG oligodeoxyribonucleotide (ODN) as adjuvant on the immune responses in PBMC and CD4+ T cell with chronic hepatitis B virus.
METHODSThe selected 20 infections were averagely divided two groups. The frequency of IFN-gamma secreting PBMC and CD4+ T cell in immune tolerant phase and in the immune clearance phase that had stimulated by CpG ODN, HBsAg and Mixture [CpG ODN + HBsAg] were analyzed by enzyme linked immune spot (ELISOT).
RESULTSThe PBMC and CD4+ T cell were differently incubated by CpG ODN, HBsAg and M [CpG ODN + HBsAg]. The number of IFN-gamma spot differently are 3 +/- 8, 339 +/- 429, 375 +/- 496, 1 +/- 4, 5 +/- 16 and 5 +/- 12; the results of immume tolerance are 3 +/- 8, 361 +/- 153, 375 +/- 276, 0 +/- 2, 2 +/- 2 and 4 +/- 4; but the results of immune clearance are 3 +/- 21, 289 +/- 345, 405 +/- 656, 2 +/- 14, 8 +/- 40 and 7 +/- 30. The IFN-gamma spots statistical analysis of PBMC were differently incubated by HBsAg and M, the total is P = 0.720, The IFN-gamma spots statistical analysis of CD4+ T cell were differently incubated by HBsAg and M, the total is P = 0.890, The IFN-gamma spots statistical analysis of PBMC and CD4+ T cell were differently incubated by M, the total is P = 0.000.
CONCLUSIONSThe ability that CpG ODN can not significantly increase the IFN-gamma secreting of PBMC and CD4+ T cell that were incubated by HBsAg to the infection in immune tolerant phase and in the immune clearance phase, but the PBMC outweighed The CD4 T cell.
