The immunostimulatory study on peripheral blood mononuclearcell and CD4+ T Lymphocyte of HBV infections that were activated by CpG oligonucleotide
10.3760/cma.j.issn.1003-9279.2008.04.013
- VernacularTitle:CpG ODN激活慢性HBV感染者外周血单个核细胞和CD4+T细胞功能的研究
- Author:
Tian-Xin XIANG
1
;
Xiao-Ping WU
;
Ming-Hui LI
;
Yao XIE
;
Jia-Gan HUANG
;
Xiao-Yan MA
;
Xiao-Quan LI
;
Bin ZHANG
Author Information
1. 北京地坛医院
- Keywords:
Oligonucleotide array sequence analysis;
Hepatitis B,chronic;
Monocytes;
CD4+ positive T- Lymphocytes
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(4):278-280
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of CpG oligodeoxyribonuclentide (ODN)as adjuvant on the immune responses in PBMC and CD4+ T cell with chronic hepatitis B virus. Methods The selected 20 infections were averagely divided two groups. The frequency of IFN-γ secreting PBMC and CD4+ T cell in immune tolerant phase and in the immune clearance phase that had stimulated by CpG ODN, HBsAg and Mixture[CpG ODN+HBsAg] were analyzed by enzyme linked immune spot(ELISOT). Results The PBMC and CD4+ T cell were differently incubated by CpG ODN+ HBsAg and M[CpG ODN + HBsAg]. The number of IFN-γ spot differently are 3±8, 339±429, 375±496, 1±4, 5±16 and 5±12;the results of immume tolerance are 3±8,361±153, 375±276, 0±2, 2±2 and 4±4; but the results of immune clearance are 3±21, 289±345, 405±656, 2±14, 8±40 and 7±30. The IFN-γ spots statistical analysis of PBMC were differently incubated by HBsAg and M,the total is P = 0.720, The IFN-γ spots statistical analysis of CD4+ T cell were differently incubated by HBsAg and M, the total is P = 0. 890, The IFN-γ spots statistical analysis of PBMC and CD4+ T cell were differently incubated by M, the total is P=0.000. Conclusions The ability that CpG ODN can not significantly increase the IFN-γsecreting of PBMC and CD4+ T cell that were incubated by HBsAg to the infection in immune tolerant phase and in the immune clearance phase, but the PBMC outweighed The CD4+ T cell.
