Expression and purification of TAT-HBX-EGFP fusion protein and its transmembrane distribution in mouse.
- Author:
Ying SHI
1
;
Fei-Li WEI
;
Ya-Li LIU
;
Yun-Xia JI
;
Hao WU
;
De-Xi CHEN
;
Yu-Sen ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Membrane; genetics; metabolism; Escherichia coli; genetics; metabolism; Female; Gene Expression; Green Fluorescent Proteins; genetics; isolation & purification; metabolism; Hepatitis B; metabolism; virology; Humans; Liver; metabolism; Male; Mice; Mice, Inbred ICR; Protein Transport; Recombinant Fusion Proteins; genetics; isolation & purification; metabolism; Trans-Activators; genetics; isolation & purification; metabolism; Viral Regulatory and Accessory Proteins; genetics; isolation & purification; metabolism; tat Gene Products, Human Immunodeficiency Virus; genetics; isolation & purification; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(4):287-289
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo highly express TAT-HBX-EGFP fusion protein and study its distribution in mouse liver.
METHODSTAT-HBX-EGFP recombinant vector was constructed and fusion protein was induced by IPTG and expression in BL21; fusion protein was purified by Ni-NTA argarose, then injected into the peritoneal cavity of the mice. Distribution of fusion protein was observed by immunofluorescence.
RESULTSTAT-HBX-EGFP was highly expression in E. coli; HBX could be induced into mouse liver by TAT.
CONCLUSIONHBX protein could be induced into mouse liver by TAT induced peptide.
