Establishment of a prion disease PrPSc panel from the brain tissues of experimental hamsters infected with scrapie agent 263K
10.3760/cma.j.issn.1003-9279.2008.05.001
- VernacularTitle:朊病毒病实验动物脑组织PrPSc样本库的建立
- Author:
Bao-Yun ZHANG
1
;
Chan TIAN
;
Jun HAN
;
Chen GAO
;
Qi SHI
;
Hui-Ying JIANG
;
Wei ZHOU
;
Xiao-Ping DONG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Prions;
Serapie;
PrPC proteins
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(5):321-323
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a prion disease PrPSc panel from the brain tissues of experimental hamsters and to address the stability of the panel conserved under the specific condition, for evaluating the diagnostic techniques of human and animal's prion diseases. Methods 30 brain tissues of hamsters infected with scrapie strain 263K intracerebrally and 30 ones of normal hamsters were enrolled in this panel. Each brain sample was prepared to 10%, 1% and 0.5% homogenates and aliquoted into stocks. The presences of PrPSc in each brain sample were evaluated with PrP-specific Western Blots and partially with immunohistochemistry, and the stabihty of PrPSc signals in each sample were repeatedly assessed half a year later and 3 years later. Results PrPSc signals were detected in all stocks of 10% brain homogenates from infected hamsters, 26 out of 30 stocks of 1% homogenates and 19 out of 30 stocks of 0.5 % homogenates. The assessments of PrPSc signals in all samples half-year and three years later demonstrated almost unchanged. All homogenates of brain tissues of normal hamsters were PrPSc negative. Conclusion A prion disease PrPSc panel of the brain tissues, which includes 90 PrPSc positive stocks and PrPSc negative ones, was successfully estabhshed, with a reliable stability of PrPSc signals.
