Investigation the risk of two model virus in the laboratory
10.3760/cma.j.issn.1003-9279.2008.05.003
- VernacularTitle:两种模式病毒灭活风险实验研究
- Author:
Yuan LI
1
;
Guo-Yong MEI
;
Hui-Ying JIANG
;
Gui-Rong WANG
;
Chan TIAN
;
Lu HAN
;
Wei-Fang HAN
;
Qiang WEI
;
Yong-Yun ZHOU
;
Gui-Zhen WU
;
Ke-Xia WANG
;
Jun HAN
Author Information
1. 安徽理工大学医学院
- Keywords:
Biosafety;
Model virus;
Disinfectants;
Inactivation
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(5):327-329
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the survival time of recombination rival in environment and inactivation ability of different disinfectant and ultraviolet radiation against virus. Methods NC membranes absorbed the recombinant adenovirus (rADV) or herpes simplex vires (rHSV) with green fluorescence protein (GFP) were laid, or immersed in various concentration of different disinfectants such as ethanol, sodium hypochlorite, lysol and geramine and then taked out them every 15 min, or exposed under ultraviolet radiation, then the NC membranes were adsorbed 1 h in cell, 37 ℃ 5 % CO248 h. The results were observed under the fluorescence microscope. Results (1) the average survival time of rHSV under environment is less than 60 min, rADV is almost up to 2 h. (2) The infection ability of rHSV and rADV was inactived 15 min by both ethanol(100%, 70% and 50%) and sodium hypochlorite (5%, 2.5% and 1.25%). (3) Two virus can be killed by 0.1% bromngeramine. (4) Both 5% and 2.5% lysol, but rADV can not lost the infection on Vero Cell until 75 min by 1.25% Lysol. (5) The rHSV was inactivated under ultraviolet radiation, but rADV was not. Conclusion The survival time of is different from beth envelope rival and the no-envelope viral under nature environment and the inactivate ability of disinfectant also is different between two model virus; Disinfectant should be choose according to virus type.
