HBV transinfected enhances the PD-L expression of cytokines stimulating.
- Author:
Xue-jie WU
1
;
Ji CHEN
;
Man-hua ZHU
;
Yuan HONG
;
Lu ZHONG
;
Gui-qiang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cytokines; genetics; metabolism; Gene Expression; Hepatitis B; metabolism; pathology; Hepatitis B virus; Hepatocytes; metabolism; virology; Humans; Liver; pathology
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(5):333-335
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether the PD-L expression in the liver cell lines transinfected with HBV (HepG2.2.15 cells) can be up-regulated after cytokines stimulating.
METHODSTo apply the liver cell lines (HepG2 cells and HepG2.2.15 cells) as a model, the cells were stimulated with IL-4, IFN-alpha and IFN-gamma (final concentration were 10 ng/ml, stimulated for 12 hours) and RT-PCR was carried out to determine the PD-L expression before and after cytokines stimulating.
RESULTSWhether or not transinfected with HBV, IFN-alpha and IFN-gamma both can induce the liver cell lines (HepG2 cells and HepG2.2.15 cells) PD-L1 expression while IL-4 can not; IL-4, IFN-alpha, IFN-gamma all can induce the PD-L2 expression in HepG2.2.15 cells which was transinfected with HBV, only IFN-gamma can induce the PD-L2 expression in HepG2 cells which was not transinfected with HBV.
CONCLUSIONIFN-alpha, IFN-gamma both can induce the PD-L1 expression in HepG2 cells and HepG2.2.15 cells, while it is easy for cytokines to induce the PD-L2 expression in HepG2.2.15 cells which was transinfected with HBV, this may provide a potential mechanism of the molecular basis for chronic HBV infection.
