HBV transinfected enhances the PD-L expression of cytokines stimulating
10.3760/cma.j.issn.1003-9279.2008.05.005
- VernacularTitle:HBV转染增强细胞因子上调PD-L表达
- Author:
Xue-Jie WU
1
;
Ji CHEN
;
Man-Hua ZHU
;
Yuan HONG
;
Lu ZHONG
;
Gui-Qiang WANG
Author Information
1. 北京大学第一医院
- Keywords:
Cytokinins;
Hepatitis B virus;
PD-L1;
PD-L2
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(5):333-335
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the PD-L expression in the liver cell lines transinfected with HBV(HepG2.2.15 cells) can be up-regulated after cytokines stimulating. Methods To apply the liver cell lines (HepG2 cells and HepG2.2.15 cells) as a model, the cells were stimulated with IL-4, IFN-α and IFN-γ (final concentration were 10 ng/ml, stimulated for 12 hours) and RT-PCR was carried out to determine the PD-L expression before and after cytokines stimulating. Results Whether or not transinfected with HBV, IFN-α and IFN-γ both can induce the liver cell lines (HepG2 cells and HepG2.2.15 cells) PD-L1 expression while IL-4 can not; IL-4, IFN-α, IFN-γ all can induce the PD-L2 expression in HepG2.2.15 cells which was transinfected with HBV, only IFN-γ can induce the PD-L2 expression in HepG2 cells which was not transinfected with HBV. Conclusion IFN-α, IFN-γ both can induce the PD-L1 expression in HepG2 cells and HepG2.2.15 cells, while it is easy for cytokines to induce the PD-L2 expression in HepG2.2.15 cells which was transinfeeted with HBV, this may provide a potential mechanism of the molecular basis for chronic HBV infection.
